Solubilization of Peptides

ity. The amino acid composition will provide a fi rst indication: before choosing a solvent, the sequence should be studied and the number of acidic, basic and neutral residues should be determined. The side-chain functionalities of acidic and basic amino acids will be charged at physiological pH. The nature of the Nand the C-terminal functionality has to be taken into consideration. The neutral amino acids can be roughly divided into two categories, non-polar, i.e. more or less hydrophobic residues and polar residues. As “dispersed” proline residues disrupt secondary structures, they can increase the solubility.